Application 9001

Recycling Novozym® 435 with a rotating bed reactor in the EasyMax™ 102

Investigating reactions can easily grow from an idea into very time-consuming projects, but the upside of properly understanding the reaction is great. The choice of equipment has a very large impact on the efforts required. The rotating bed reactor is a tool that unlocks the full potential of your Mettler-Toledo EasyMax™ 102 Advanced synthesis workstation for this development.

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The rotating bed reactor protects the solid-phase catalysts from mechanical damage, which prolongs the catalyst lifetime. This increase in stability and the greatly simplified transfer of the catalyst between batches makes lab work a joy. In this application note, the popular immobilized CalB Novozym® 435 was reused in 7 reaction cycles without activity loss.

No filtration steps were needed between cycles or for the samples extracted for analysis. Washing the resin between runs was fast, simple and robust, without running the risk of material loss.

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Conditions: A SpinChem® S2 RBR containing Novozym® 435 (1 g), was rotated at 500 rpm in a preheated (30 °C) substrate solution containing 2-Ethyl-1-hexanol (5.21 g), Vinyl acetate (3.44 mL) and heptane (110 mL). The reaction was repeated for seven cycles, and samples for each run were taken after 0, 10, 20, 30 and 60 min. Analysis of 2-Ethylhexyl acetate product was done suing GC-FID after 1:9 dilution in heptane containing internal standard (tetradecane, 50 mmol). Between cycles, the resin was washed by spinning the RBR in heptane for 1 min.

Recycling Novozym® 435 with a rotating bed reactor in the EasyMax™ 102

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