“In this study, the single and combined effects of water activity, temperature and substrate ratio were studied on the reaction between high oleic sunflower oil and ethyl stearate producing 1,2-stearin-3-olein (SOS) using immobilized lipase from Rhizopus oryzae (Lipase DF “Amano” IM). […] To set the water activity, a control system based on dry/humid nitrogen gas sparging was developed. The models were found to adequately describe the effect of the factors on product yield and quality as well as being robust.”
Highlights:
- "Water is an important factor in enzymatic reactions as it affects the enzyme activity and the equilibrium position of hydrolytic reactions. To study these effects, the authors first developed a system based on relatively simple and low-cost components that could continuously control the activity of water in organic media in a SpinChem® RBR."
- “Increased temperature and water activity had initial positive influence that shifted to substrate ratio and a negative influence of water activity as equilibrium was approached.”
- “Product quality was mostly affected by temperature negatively, as an increased temperature promoted acyl migration.”
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